Analysis of duodenal iron content and ferritin expression of mice with tissue-specific versus total IRP2 deficiency. (A) Relative total nonheme iron levels and (B) Western blot analysis of ferritin H- (indicated FTH1) and L- (indicated FTL) chain expression in the duodenum of Ireb2^Δ/Δ mice lacking IRP2 expression in all tissues (total) versus Ireb2^+/+ littermates, and in Ireb2^VilCre, Ireb2^AlfpCre, and Ireb2^LysMCre mice with selective IRP2 ablation in intestinal epithelial cells (IECs), in hepatocytes (hepat.), or in macrophages (macro.), respectively, versus their corresponding control littermates; the presence (+) or absence (−) of the Cre transgene is indicated. (A) Data are presented as average plus or minus SEM. Within each group, the iron level of control mice was set to 100%. Top histogram represents male mice; bottom, female mice. The number of animals per group (n) is indicated. P: Student t test. (B) A representative Western blot experiment is presented. β-Actin was used as a loading control. Vertical lines have been inserted to indicate repositioned gel lanes.