In vitro–generated BCR/ABL-expressing DCs have a low maturation status. (A-D) DCs were generated in vitro from BCR/ABL-GFP or empty vector GFP–transduced H8 bone marrow cells. Immature (A), LPS-matured (B), and αCD40-maturated (C) BCR/ABL-expressing and control DCs were analyzed for MHC class II^high molecules, CD86, CD80, CD83, and CD70 expression by flow cytometry. Dot plots are gated on GFP-expressing cells. Numbers indicate the percentage of CD11c⁺ DCs expressing the stained molecule. (D) Summary of data in panel B of 3 independent experiments is shown as percentage (mean ± SEM) of MHC class II^high, CD86⁺, CD80⁺, CD83⁺, or CD70⁺ cells within total GFP⁺CD11c⁺ cells: (■) represents BCR/ABL-expressing DCs; (□), empty GFP vector–transduced control DCs, LPS-matured; ns indicates not significant.