BCR/ABL-expressing DCs have a low maturation status in vivo. (A-C) Spleen, thymus, and blood of CML and naive C57BL/6 mice were isolated. The expression of MHC class II molecules (A), CD86 (B), and CD80 (C) on BCR/ABL-GFP-expressing CD11c⁺ DCs in CML mice and on CD11c⁺ DCs from naive C57BL/6 mice (open histograms) was compared with isotype control stainings (filled histograms). One representative FACS plot of 3 independent experiments is shown. (D) Fold increase of mean fluorescence intensity of stainings of CML mice compared with naive C57BL/6 mice. Pooled data from 3 independent experiments with a total of 7 CML mice and 3 C57BL/6 mice are shown; ns indicates not significant.