CML model and BCR/ABL-expressing granulocytes and DCs in different organs. (A) Survival of CML (—) and control mice (, empty GFP vector–transduced bone marrow) is shown in Kaplan-Meier plots. (B) Blood smear (top) and cytospin analysis (bottom) of CML mice and naive C57BL/6 mice. One representative Pappenheim staining of 10 is shown (original magnification ×60) included in “Methods.” (C) Spleen size of naive C57BL/6 mice and CML mice. (C,D) Bone marrow, thymus, spleen, inguinal and mesenterial lymph nodes (LN), and blood were isolated from CML mice. Samples were analyzed for the presence of BCR/ABL-GFP–expressing GR-1⁺ granulocytes (D) and CD11c⁺ DCs (E) by flow cytometry. Numbers indicate the percentage of BCR/ABL-expressing cells of total GR-1⁺ or CD11c⁺ cells. One representative FACS plot of 4 independent experiments is shown.