Generation of VASH1 and VASH2 knockout mice and their steady state subcutaneous vascular architecture. (A) VASH1 and VASH2 knockout mice were generated as described in “Generation of VASH1 and VASH2 knockout mice.” Genotyping and the analysis of each transcript by RT-PCR are shown. (B) Ear skin was used to show the steady state vascular architecture of ear skin. Top panels show immunostaining of CD31 (green) and LYVE-1 (red). Bottom panels show SEM of capillary vessels.