Biochemical properties of scFv-Fc-Rnase. (A) Plasmon resonance. Overlay plot of various concentrations (scFv-Fc-RNase, 6.6–33 nM) were applied on CM5 chip with immobilized recombinant CD30-Fc. Samples volumes of 50 μL were injected at a flow rate of 25 μL/minute. Control flow cell was immobilized with lysozyme to observe unspecific protein protein interaction. (B) Size exclusion chromatography on a calibrated Superdex-200. The single peak at 13.03 mL corresponds to a molecular mass of 150 kDa. Fractionated samples were analyzed by SDS-PAGE (10%; reducing conditions, silver staining).