Schematic setup of different CD30-specific antibody and huTR constructs generated. Signal peptides for yeast (■) and mammalian expression (▒). V_H and V_L indicate the variable domains of the CD30-specific scFv antibody fragment; C_L and C_H, constant immunoglobulin domains; H, IgG₁ hinge region; L1, (Gly₄-Ser)₃ linker between V_H and V_L of the scFv fragment; L2, 6–amino acid linker AAASSG for antibody RNase fusion; tags, tags for purification and detection; and stop codon (◆). Indicated restriction sites were used for cloning into expression vectors and allow modular exchangeability of huTR elements. Constructs are not drawn to scale.