Low amount of mTCC was sufficient to down-regulate the proinflammatory effect of SC5b-9. (A) mTCC was assembled on mC7 incubating HUVECs with increasing amount of C5b-6 (0.5 μg [a], 1 μg [b], 2 μg [c], and 3 μg [d] to a final volume of 100 μL). C8 and C9 were added to C5b6 at a molar ratio of 1:2:2, respectively. The assembly of mTCC was revealed by ELISA on whole cells using anti-C9 neoantigen. (B) HUVECs were stimulated with SC5b-9 (5 μg/mL) either alone or in the presence of mTCC assembled using the same quantity of C5b6, C8, and C9 as used above. The expression of VCAM-1 was evaluated by ELISA on whole cells as indicated in the legend to Figure 6A. Values are mean (± SD) of triplicate determinations of 3 separate experiment (*P < .05; **P < .01 vs complete cell-culture medium).