Effect of soluble ephrinB2 on tumor growth. (A) A soluble form of ephrinB2 (ephrinB2-Fc) inhibited subcutaneous tumor growth in a mouse tumor models with KLN205. (B) Hematoxylin and eosin (H.E.) staining and immunohistologic staining for factor VIII–related antigen and for hypoxia (hypoxyprobe-1 kit). (C,D) The quantitative analyses of histologic data. The factor VIII–positive vessel area (C) and hypoxic area (D) were measured, and the percentages of the area in HPF (×100) were compared. Data are mean plus or minus SE (n = 7 per group). *P < .05 versus control by Student unpaired t test.