Effect of HMD4-2 on tumor angiogenesis and hypoxia. (A,C) Hematoxylin and eosin (H.E.) staining and immunohistologic staining for factor VIII–related antigen and for hypoxia (hypoxyprobe-1 kit) of KLN205 tumors (A) and LLC tumors (C). The hypoxic area was stained brown. (B,D) The quantitative analyses of histologic data of KLN205 tumors (B) and LLC tumors (D). The factor VIII–positive vessel area and hypoxic area were measured, and the percentages of the area in HPF (×100) were compared in each mouse tumor model. Data are mean plus or minus SE (n = 7 per group). *P < .05 versus control by Student unpaired t test or 1-way ANOVA with Fisher least-significant-difference test.