Gene expression analysis of tumors by PCR at different points in time. (A,B) In real-time PCR analysis, multiplex reactions were run in triplicates, and samples were normalized to the housekeeping gene HPRT. (A) Relative expression of genes of interest is represented as fold differences of the mean of Th1 cell–treated mice (d10, n = 10; d11, n = 10; d13, n = 10; d18, n = 9) over PBS controls (d10, n = 10; d11, n = 10; d13, n = 8; d18, n = 9), with highest levels on day 13 (*P < .05). (B) IL-17 expression in tumors of Th1 cell–treated mice (d10, n = 10; d13, n = 8; d18, n = 9) and control mice (d10, n = 10; d13, n = 7; d18, n = 12) on days 10, 13, and 18, showing an increase of IL-17 in tumors of Th1 cell–treated mice from day 13 on (*P < .05).