The Rab27a effector MyRIP is present on more mature WPBs. (A) Confocal images acquired of immunofluorescence staining of MyRIP (green), and VWF (red) inset magnified image of polarized localization of MyRIP. Boxed images show magnified images of VWF expression (B,D) and MyRIP (C,E) in immature (B,C) and more mature WPBs (D,E). (F) HUVECs were transfected with MyRIP-GFP, and a maximum intensity projection was made of confocal images acquired of a cell expressing low levels of MyRIP-GFP and VWF (red). (G-J) HUVECs were transfected with MyRIP-GFP, and a maximum intensity projection was made of confocal images acquired of a cell expressing high levels of MyRIP-GFP and VWF (blue) alongside rhodamine-conjugated phalloidin (red). Boxed square shows magnified images of phalloidin (H), MyRIP-GFP (I), and VWF (J). Scale bars represent 10 μm.