Improved engraftment in STAT5ab^+/null host mice by Gab2 deletion during nonablative transplantation. Recipient mice (CD45.2) were transplanted with 5 × 10⁶ CD45.1⁺ BM cells. (A) Percentage of donor chimerism (% CD45.1⁺ cells) in each recipient mouse 16 weeks after BM injection. From 2 separate injection dates, wild-type (n = 9), STAT5ab^+/null (n = 8), Gab2^−/− (n = 9), and from 5 separate injection dates STAT5ab^+/nullGab2^−/− (n = 14). The Wilcoxon 2 sample test was used for statistical analysis. Numbers 1 to 3 are mice that were further analyzed in panel B. (B) Representative dot plots of primary recipient mice as well as secondary transplanted recipients 16 weeks after BM injection and the percentage of donor-derived Gr-1, B220, Ter119, or CD4 cells are shown. In panel A, numbers 2 and 3 denote specific mice that were killed and BM analyzed by secondary transplantation (one donor into 5 recipients for Gab2^−/−STAT5ab^+/null), and average plus or minus SD values are indicated above each plot.