Anti-CD38 stimulation promotes T2 B lymphocyte differentiation. (A) T2 subsets, obtained as in Figure 2, were preincubated 30 minutes with 8-Br-cADPR or medium and activated with rat-IgG2a (50 μg/mL), anti-CD38 (50 μg/mL), or anti-IgM (10 μg/mL). Cells were harvested at 24 or 48 hours and restained to evaluate differentiation. (B) T2 B cells were preincubated as in panel A, activated with rat-IgG2a or anti-CD38 at (25 μg/mL) or anti-IgM (5 μg/mL; “Proliferation, diferentiation, and apoptosis assays”) for 48 hours, and restained as in panel A. Numbers in each dot plot indicate the percentage of cells and are representative from 3 independent experiments with similar results.