Figure 5
Figure 5. Differential p38MAPK phosphorylation and p21 induction upon acute stress in HO-1+/− HSCs and HPCs. (A) Mice were bled retro-orbitally to cause an approximately 10% blood loss, followed by heme challenge (50 μmol/kg, intraperiotoneally) to mimic a hemolysis. BM cells were prepared for immunostaining using antibodies against SLAM family members (CD150, CD48, and CD244) for measurement of levels of intracellular HO-1 protein (B) and ROS (C), phosphorylated p38MAPK (D), and p21 induction (E) within HSCs (CD150+CD48−CD244−), MPPs (CD244+CD48−CD150−), and more restricted progenitors (CD48+CD244+CD150−). Values are the mean percentages indicating the gated proportion of cells. Please note that some of the histograms were skewed to the left because of the number of events on the y-axis.

Differential p38MAPK phosphorylation and p21 induction upon acute stress in HO-1+/− HSCs and HPCs. (A) Mice were bled retro-orbitally to cause an approximately 10% blood loss, followed by heme challenge (50 μmol/kg, intraperiotoneally) to mimic a hemolysis. BM cells were prepared for immunostaining using antibodies against SLAM family members (CD150, CD48, and CD244) for measurement of levels of intracellular HO-1 protein (B) and ROS (C), phosphorylated p38MAPK (D), and p21 induction (E) within HSCs (CD150+CD48−CD244−), MPPs (CD244+CD48−CD150−), and more restricted progenitors (CD48+CD244+CD150−). Values are the mean percentages indicating the gated proportion of cells. Please note that some of the histograms were skewed to the left because of the number of events on the y-axis.

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