Chim3 inhibits HIV-1 replication analogously to F12-Vif. LV-transduced CB-derived CD4⁺ T lymphocytes were infected in triplicate cultures with either the X4 NL4-3 (A) or the R5 AD8 (B) and AD1 (C) molecular clones or the R5 laboratory-adapted BaL (D) HIV-1 strains at an MOI of 0.1. Culture supernatants were harvested every 4 days, stored at −20°C, and then assessed for RT activity.