Expression levels of oncogenes in T lymphocytes and thymocytes after transplantation of transduced HSCs/HPCs and mature T cells. Animals were killed 6 weeks after transplantation, and Western blot analysis was performed for the expression of LMO2-HA (A,B), TCL1 (C), and ΔTrkA (D) in thymocytes of HSCs/HPCs and T lymphocytes of TC-transplanted animals. Lymphocytes of MP91-eGFP–transduced mature T cells and thymocytes of MP91-eGFP-transduced progenitor cells were used as negative controls. eGFP expression served as a loading control to ensure equal quantities of gene-modified cells. For MP91-LMO2-HA–transduced cells, flow cytometric analysis of lymphocytes and thymocytes of TC- and HSC/HPC-transplanted animals showed comparable expression intensities (B). In panel A, A1-3 indicates number of animals killed.