IL-25R is expressed by human blood CD14⁺ cells. (A) Representative dot-plots showing IL-25R in human PBMCs isolated from healthy volunteers and simultaneously analyzed for the expression of IL-25R, CD3, CD14, CD19, and CD56. Numbers in the selected areas indicate the percentages of CD3, CD14, CD19, and CD56 cells positive for IL-25R. One of 6 representative experiments in which similar results were obtained is shown. (B) CD16⁺ cells express IL-25R. CD14⁺ cells were purified from PBMCs and stained for IL-25R and CD16. One of 3 representative experiments is shown. Right inset shows the mean fluorescence intensity (MFI) of IL-25R expression in CD14⁺ cells either positive or negative for CD16. Data indicate mean (± SD) of 3 separate experiments. (C,D) Real-time PCR data for the membrane-bound IL-25R isoform RNA transcripts in blood CD14⁺ (CD14) cells (C) and THP-1 (D). Levels are normalized to β-actin and indicate the mean (± SD) of all experiments. CD14⁺ and THP-1 experiments were performed 6 and 3 times, respectively.