Rabaptin-5 deficiency does not influence FcϵRI recycling but does alter surface stability of β1 integrin. (A) BMCMCs were induced to adhere to fibronectin (FN)-coated coverslips as described in Document S1, “Immunofluorescence,” labeled with Alexa 647-labeled α-FcϵRIα Fabs on ice, transferred to 37°C, and then fixed and processed for confocal microscopy at the indicated times. Bar represents 7.5 μm. (B) FcϵRI recycling was assessed in shC- or shR-treated BMCMCs using biotinylated α-FcϵRIα Fabs as described in Document S1, “FcϵRI recycling.” Bar graph represents data pooled from 3 separate pairs of shC- or shR-treated BMCMCs. (C) Transferrin recycling in shC- or shR-treated BMCMCs was performed as described in Document S1, “Transferrin recycling.” Bar graph represents data pooled from 4 separate pairs of shC- or shR-treated BMCMCs. (D) shC- or shR-treated BMCMCs were exposed to 50 μM of primaquine (left bar graph) or vehicle (right bar graph) for the indicated times, and then surface β1 integrin levels were assessed by flow cytometry. Data were pooled from 3 separate pairs of shC- or shR-treated BMCMCs. +P < .05; ++P < .01 for shC- versus shR-treated cells; *P < .05, **P < .01, ***P < .001, versus t = 5 minutes time point (B,C) or time 0 (D).