Rabaptin-5 deficiency does not alter intracellular FcϵRI localization to the trans-Golgi. (A) BMCMCs were processed for confocal microscopy as in Figure 1 and stained with antibodies to calnexin (red; endoplasmic reticulum) and GCC185 (green; trans-Golgi network) to localize intracellular FcϵRI (blue). Magnified regions are outlined by dashed boxes and shown in the upper right corner of each panel. (B) The relative subcellular localization of GCC-185 (green) and FcϵRI (red) was examined in control (shControl) and Rabaptin-5-deficient (shRabaptin-5) BMCMCs. Large panels show overlay of GCC-185 and FcϵRI immunostaining. Magnified images of the regions outlined by dashed boxes are shown in the upper right corner of each panel. Bars represent 7.5 μm.