KIR2DL1 promotes TCR-induced IL-2 production in a ligand-independent manner. Jurkat, EV, and JK32 cells were incubated with SEE-pulsed 721.221, 721.221/HLA-Cw4, or 721.221/HLA-Cw7 cells for 6 hours. Culture supernatants were collected, and IL-2 production was determined by ELISA. Error bars represent the SDs of n = 3 experiments.