EZH2 knock-down in MYC-dependent BL cell lines. (A) At indicated time points, cell count analysis was performed after induction of EZH2 shRNA#1 or EZH2 shRNA #2 expression in transfected Raji and Namalwa cells. After 96 hours of shRNA expression, cell numbers were significantly reduced in Raji and Namalwa cells (P = .031, exact Wilcoxon rank sum test). As a control, empty vector containing cells were treated with Dox for the same time periods. (B) Protein expression analysis of EZH2 after Dox induced shRNA expression at indicated time points. (C) Cell cycle analysis in empty vector and shRNA-transfected Raji and Namalwa cells was determined by PI staining at indicated time points after Dox treatment. Within the shRNA-expressing cell lines, there was an increased percentage of cells in G1 phase compared with the empty vector control (P = .031, exact Wilcoxon rank sum test).
