Proliferation changes after inducible miRNA expression in Namalwa. Namalwa cells transfected with an episomal Tet-inducible expression system were treated with Dox for 24 hours and 72 hours; subsequently, cell proliferation was determined and normalized to the empty control. Overexpression of miR-20a slightly increased cell counts, whereas induction of miR-489 and miR-494 caused no significant cell count alterations. In contrast, miR-181a, miR-16, and most prominently miR-26a expression resulted in reduced cell proliferation.