miRNA expression profiling in MYC-dependent lymphoma. (A) Supervised analysis for MYC-regulated miRNAs (class comparison; P < .01, paired t test with random variance model paired samples) based on miRNAs displayed in panel C. Expression levels have been set to 0 in the untreated samples to display Dox-related changes. MYC-induced miRNAs are color coded in red, whereas repressed miRNAs are depicted in green. (B) Validation of microarray data by Northern blot analysis (doxycycline treatment for 18 hours). (C) Unsupervised average linkage cluster of MYC-induced lymphoma cell lines (n = 26) based on 154 differentially expressed miRNAs. Two major cell line clusters were identified, mainly characterized by a distinct miRNA pattern (blue bar).