Real-time PCR analysis showing different gene expression patterns compared with leukemia cases with MLL translocations. Log₁₀ (Relative Quantity) is 2^−ΔΔC_T, where the endogenous control is 18S rRNA and the calibration sample (set to 1 for all genes) is bone marrow of patient at 22 months after neuroblastoma diagnosis but before MDS diagnosis, in which 100% of cells contained the translocation by karyotype analysis. No bar appears in graph for any transcript that was undetectable. Error bars indicate standard deviation in triplicate reactions. Treatment-related leukemias are specified as t-ALL or t-AML. Patients 38, 45, and t-120 have been described.³⁵