Up-regulation of DNAM-1 (PVR) and NKG2D (MICA and MICB) ligands by doxorubicin or melphalan treatment depends on DNA damage sensors ATM and ATR. The SKO-007(J3) cell line was preincubated for 2 hours with caffeine (1 mM) or KU55933 (10 μM), then treated with doxorubicin (0.05 μM) or melphalan (22 μM) for a further 48 hours. The expression of PVR, MICA, and MICB was analyzed by flow cytometry. On the y-axis is shown the median fluorescence intensity (MFI) values of ligand expression obtained by subtracting the MFI of the isotype control antibody. Results are representative of 1 of 4 independent experiments.