JAK2V617F SRCs are capable to give rise to multilineage differentiated cells. (A) Human B-lymphoid (CD45⁺CD19⁺) and myeloid (CD45⁺CD33⁺) cells engrafted in 10 NOD/SCID mice 15 weeks after transplantation were sorted and genotyped for JAK2V617F. The percentage of JAK2V617F over total JAK2 is reported. (B) Single CD34⁺CD38⁻ cells from 2 PV, 3 PMF, and one post-PV MF were cultured in B/NK/myeloid differentiation conditions. Clones with lympho-myeloid potentialities (MNK and BMNK) were genotyped. The histograms represent the numbers of lympho-myeloid clones from each patient. ▭ represents wild-type JAK2; bars, heterozygous JAK2V617F; , homozygous JAK2V617F.