The JAK2V617F mutation is present in LT-SRCs. CD34⁺CD45⁺ cells present in the mouse BM were sorted and grown in LTC-IC medium. The LTC-IC frequencies were determined (y-axis), and LTC-IC-derived erythroid and granulocytic colonies were genotyped. Each histogram represents a mouse reconstituted with PV or PMF sample. On each histogram is indicated the proportion and the number of wild-type (▭), JAK2V617F heterozygous (), and homozygous () colonies.