ICAM-1 and ICAM-5 are expressed in hippocampal astrocytes and neurons, respectively. ICAM-1 was immunostained together with GFAP (top panels) or ICAM-5 (bottom panels) in hippocampal cells, and visualized with a confocal fluorescence microscope. ICAM-1 (red) was exclusively expressed in hippocampal astrocytes as indicated by the astrocyte marker GFAP (green, top panels). Coexpression is shown in yellow. ICAM-1 was not detected in hippocampal neurons (bottom panels), whereas ICAM-5 (green, bottom panels) was exclusively expressed in hippocampal neurons, but not in astrocytes (arrows in bottom panels). Bars represent 10 μm.