Aggregation is attenuated in ILK-deficient platelets. The levels of ILK protein in control (Ctrl) ILK(+/+):MxCre and KO mice ILK(lox/lox):MxCre at 8 days after pIpC injection were assessed by immunoblotting, and a GAPDH reprobe was used to check for equal loading (A). Platelet counts in whole blood from control (Ctrl) and KO are plotted (B; n = 4, P = .98). Washed platelets from ILK KO and control (Ctrl) mice were stimulated with collagen at a final concentration of 100 μg/mL. A representative aggregation trace is shown (C), and the percentage aggregation is plotted from 3 replicate experiments (D; n = 3, mean ± SEM; ***P < .005). Platelets in PRP from ILK KO and control (Ctrl) mice were matched for platelet number and stimulated with collagen at a final concentration of 100 μg/mL and monitored for 5 minutes. A representative aggregation trace is shown (E), and the percentage aggregation (where the control = 100%) is plotted from 3 replicate experiments (F; n = 3, mean ± SEM; all **P < .05).