Figure 2
MZ B cells and MZMs are reduced, and S aureus uptake aberrant in WASP-deficient mice. Immunohistochemistry of spleen sections from WT and WASP−/− mice. (A) Schematic representation of a B-cell follicle and the MZ in the spleen. Black arrows indicate the MZ. (B) Reduced number of MZ B cells (CD1d+ B220+) and normal localization of metallophilic macrophages (MOMA-1+ Sialoadhesin+) in WASP−/− spleens relative to WT spleens. Black arrowhead represents the MZ. (C) Normal localization of red pulp macrophages (F4/80+), CD11b+ macrophages, and DCs (CD11c+). (D) Reduced number of MZMs (MARCO+ SIGN-R1+) in WASP−/− spleens compared with WT spleens. Black arrow represents MZMs in the MZ. (E) Localization of S aureus 30 minutes after intravenous injection. Note that S aureus almost exclusively colocalize with MARCO+ MZ macrophages in WT mice, whereas WASP−/− mice show aberrant localization of S aureus. White arrowhead represents the MZ. FO indicates B-cell follicle; MZ, marginal zone; RP, red pulp. Original magnifications: panels B to D, ×10; D, ×20.

MZ B cells and MZMs are reduced, and S aureus uptake aberrant in WASP-deficient mice. Immunohistochemistry of spleen sections from WT and WASP−/− mice. (A) Schematic representation of a B-cell follicle and the MZ in the spleen. Black arrows indicate the MZ. (B) Reduced number of MZ B cells (CD1d+ B220+) and normal localization of metallophilic macrophages (MOMA-1+ Sialoadhesin+) in WASP−/− spleens relative to WT spleens. Black arrowhead represents the MZ. (C) Normal localization of red pulp macrophages (F4/80+), CD11b+ macrophages, and DCs (CD11c+). (D) Reduced number of MZMs (MARCO+ SIGN-R1+) in WASP−/− spleens compared with WT spleens. Black arrow represents MZMs in the MZ. (E) Localization of S aureus 30 minutes after intravenous injection. Note that S aureus almost exclusively colocalize with MARCO+ MZ macrophages in WT mice, whereas WASP−/− mice show aberrant localization of S aureus. White arrowhead represents the MZ. FO indicates B-cell follicle; MZ, marginal zone; RP, red pulp. Original magnifications: panels B to D, ×10; D, ×20.

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