Increased VEGF-A and PlGF protein levels without microvascular leakage in transgenic mice. Real-time TaqMan RT-PCR analysis of VEGF-A expression from total RNA isolated from ears of K14-HIF-1αDPM transgenic mice (DPM) and nontransgenic controls (NTG), using histone 3.3A as a reference (A), at baseline “C” and after 6 hours of TPA treatment “T.” VEGF-A (B) and PlGF (C) ELISA assays reveal significantly higher protein expression in untreated and TPA treated transgenic ears compared with nontransgenic controls. Lack of differential microvascular leakage, evidenced by ear Evans blue dye content, in transgenic vs nontransgenic mice (D). Error bars represent mean plus or minus SEM of 4 to 6 mice analyzed per group (*P < .05, Student t test).