American Society of Hematology

Figure 5

$Figure 5. DCs generated from CLPs in response to TLR9 ligation are functional. CLPs were sorted from CpG-injected mice and cultured under conditions designed to support B lymphopoiesis. Harvested cells were then evaluated after 8 days by flow cytometry. (A) B220+CD19−CD11c+CD11b− cells were further fractionated into pDC and NK-like IKDC populations by the NK1.1 marker, whereas B220−CD19−CD11c+CD11b+ cDCs were NK1.1−. (B) The 3 categories of DCs were sorted, and their competence to respond to CpG stimulation by production of cytokines was evaluated.$

DCs generated from CLPs in response to TLR9 ligation are functional. CLPs were sorted from CpG-injected mice and cultured under conditions designed to support B lymphopoiesis. Harvested cells were then evaluated after 8 days by flow cytometry. (A) B220⁺CD19⁻CD11c⁺CD11b⁻ cells were further fractionated into pDC and NK-like IKDC populations by the NK1.1 marker, whereas B220⁻CD19⁻CD11c⁺CD11b⁺ cDCs were NK1.1⁻. (B) The 3 categories of DCs were sorted, and their competence to respond to CpG stimulation by production of cytokines was evaluated.

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