TNF cell death responses in human myeloid leukemia cells with cFLIP targeted by siRNA. (A) THP-1 and HL60 were untransfected, transfected with control siRNA, or with HO-1 siRNA, Nrf2 siRNA, and 2 cFLIP siRNA for 24 hours Protein expression for cFLIP was measured by Western blot analysis. (B) THP-1 cells and HL60 cells were transfected with control siRNA or cFLIP siRNA-2 before treatment with 10 μM BAY 11-7082, or 10 ng/mL TNF, or BAY 11-7082 plus TNF for 24 hours. Cell number was then assessed by MTS assay as described in “Proliferation/death and apoptotic assays.” Values represent mean plus or minus SEM from 3 independent experiments.