Localization of GEs in oxygenated band 3 knockout erythrocytes. Confocal images of band 3 null erythrocytes stained for both RNA (to identify reticulocytes, green stain) and GEs (red stain), as indicated. A merger of both stains is shown in the third row (overlay), and bright field images are displayed in the bottom row. Note that even the very few mature erythrocytes present (negative for SytoRNA stain, green color) do not show enzyme localization at the membrane.