Figure 2
Gr-1+CD11b+F4/80+ cells from RMA-S tumor-bearing mice suppress T-cell proliferation. Sorted Gr-1+CD11b+F4/80+ (A) or Gr-1+CD11b+F4/80− (B) cells isolated from tumor-bearing mice on day 21 after tumor cell inoculation were cocultured with splenocytes (spl) from naive mice for 72 hours with ConA at the indicated ratios. Proliferation was assessed by 3H-thymidine incorporation assay. Data show mean plus or minus standard deviation (SD) of triplicate cultures. Asterisks indicate statistical significance (P < .01) determined by Student t test. Representative data selected from 3 independent experiments are shown.
