Figure 6
![Figure 6. Sialylated difucosylated neolactododecaosylceramide from human leukocytes. The most potent E-selectin receptor glycosphingolipid was purified by preparative HPLC and subjected to MSn analysis to determine the relative distribution of fucose residues on the poly-LacNAc backbone. The major species, representing more than 95% of the isobaric structures present, is NeuAcα2-3Galβ1-4GlcNAcβ1-3[Galβ1-4(Fucα1-3)GlcNAcβ1-3]2[Galβ1-4GlcNAcβ1-3]2Galβ1-4GlcβCer (IUPAC abbreviation: XII3NeuAc,VII3Fuc,IX3Fuc-nLc12Cer). Values are the means plus or minus SD for 5 isobaric distributions, each calculated from the relative abundances of different diagnostic fragment ions (Figure S3).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/112/9/10.1182_blood-2008-04-149641/5/zh80180823890006.jpeg?Expires=1723730802&Signature=QYVmJA~0VbQa-KMC8I-4KF9D-h8~dyx-O4mZ9wmflQEu7KE2gk04qgOUfN6KJKG11bAWcQ6SiCy556x8LuMn~oOFBdnVX6xw4A5gF04gKAoJo0rmCP2U82EOr8lAK9-S1O5o5fN~x5YEQNo8UVDvNwtupgR3C7WVWyKBQeDKmokWFDq~5cH1fPBJAwdbsWWxICJ8tyaRZNRizWxZDPdtlYVkn2QLS0UBeLDsSCf9JjdKNZWLdDghMunSrhQJBefiqXgkaAGI8znSYo~DcfBsjvqVoyuwKwrVxlUhkpSulW9Lg4rBAH3gYJSZtDM3rAU6Lo6BKHWF8LugjsFv--ks1g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Sialylated difucosylated neolactododecaosylceramide from human leukocytes. The most potent E-selectin receptor glycosphingolipid was purified by preparative HPLC and subjected to MSn analysis to determine the relative distribution of fucose residues on the poly-LacNAc backbone. The major species, representing more than 95% of the isobaric structures present, is NeuAcα2-3Galβ1-4GlcNAcβ1-3[Galβ1-4(Fucα1-3)GlcNAcβ1-3]2[Galβ1-4GlcNAcβ1-3]2Galβ1-4GlcβCer (IUPAC abbreviation: XII3NeuAc,VII3Fuc,IX3Fuc-nLc12Cer). Values are the means plus or minus SD for 5 isobaric distributions, each calculated from the relative abundances of different diagnostic fragment ions (Figure S3).
