American Society of Hematology

Figure 3

Binding of mAbs measured by flow cytometry to resting platelets and CHO cells transfected with wild-type or mutant αIIbβ3. (A) Histograms showing increased PAC-1 binding to resting platelets from patients II.4 and III.5 versus control, but no difference of fibrinogen binding or P-selectin surface expression. (B) Histograms showing similar expression levels of αIIbβ3 (with antibody PL2-73) in all CHO cell lines and increased PAC-1 binding to the αIIbβ3-H723 and αIIbβ3-A723 (reversed in the presence of 1 mM RGDS). Irrelevant mouse IgG was used to determine nonspecific antibody binding.

Binding of mAbs measured by flow cytometry to resting platelets and CHO cells transfected with wild-type or mutant α_IIbβ₃. (A) Histograms showing increased PAC-1 binding to resting platelets from patients II.4 and III.5 versus control, but no difference of fibrinogen binding or P-selectin surface expression. (B) Histograms showing similar expression levels of α_IIbβ₃ (with antibody PL2-73) in all CHO cell lines and increased PAC-1 binding to the α_IIbβ₃-H723 and α_IIbβ₃-A723 (reversed in the presence of 1 mM RGDS). Irrelevant mouse IgG was used to determine nonspecific antibody binding.

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