Images of the blood and platelets of the propositus. (A) Light microscopy (×50) of a blood smear with Romanovski stain; platelets are indicated by arrowheads. (B) Flow cytometric scatter plot analysis of platelet RNA content of propositus and control (mean volume as forward scatter and RNA content as fluorescence intensity). The immature platelet fraction is indicated by a green arrowhead, and the mature fraction is indicated by a blue arrowhead. (C,D) Electron micrographs of the propositus's platelets with immunogold staining for α_IIbβ₃ (C) and GPIb-IX-V (D). Inserts are a ×7 amplification.