Figure 1
Hierarchical NKT-cell cytokine production. (A) NKT cells were stimulated by exposure to plate-bound CD1d molecules pulsed with the indicated concentrations of α-GalCer, and cytokine secretion was determined by ELISA. (B) Plate-bound CD1d was pulsed with 1 μg/mL α-GalCer or OCH. (C-E) NKT cells were stimulated by the indicated APCs that were untreated (Autoreactive) or pulsed with 100 ng/mL of α-GalCer. (F) NKT cells were incubated with CD1d-transfected APCs for the indicated times, and GM-CSF secretion was quantitated by ELISA. Data are represented as mean plus or minus SD (not always visible on the scales shown) with amounts below the limit of detection indicated by “∼.” Panels A to E show a representative experiment performed using clone J24N.22, and in each case similar results were observed using 2 or 3 other NKT cell clones. Panel F shows 1 representative experiment of 2 performed using clone J3N.5.

Hierarchical NKT-cell cytokine production. (A) NKT cells were stimulated by exposure to plate-bound CD1d molecules pulsed with the indicated concentrations of α-GalCer, and cytokine secretion was determined by ELISA. (B) Plate-bound CD1d was pulsed with 1 μg/mL α-GalCer or OCH. (C-E) NKT cells were stimulated by the indicated APCs that were untreated (Autoreactive) or pulsed with 100 ng/mL of α-GalCer. (F) NKT cells were incubated with CD1d-transfected APCs for the indicated times, and GM-CSF secretion was quantitated by ELISA. Data are represented as mean plus or minus SD (not always visible on the scales shown) with amounts below the limit of detection indicated by “∼.” Panels A to E show a representative experiment performed using clone J24N.22, and in each case similar results were observed using 2 or 3 other NKT cell clones. Panel F shows 1 representative experiment of 2 performed using clone J3N.5.

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