Fig. 5
![Fig. 5. ADP dose response for the full platelet model. (A) Relative fluorescence of Fluo-4–loaded human platelets was measured after treatment with 100 nM, 1 μM, or 10 μM ADP. (B-F) After 1 minute of simulated rest, the platelet model was activated by setting the extracellular [ADP] to 100 nM, 1 μM, or 10 μM. Deterministic computation of (B) [Ca2+]i, (C) [IP3], (D) [PLC-β*], (E) deactivated [PLC-β], and (F) activated [Gq·GTP]. (G,H) Gel-filtered human platelets were activated with ADP and maximal changes in (G) [Ca2+]i and (H) [IP3] from basal levels were measured.36 For panels G and H, simulated responses are plotted in red.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/112/10/10.1182_blood-2008-05-157883/7/zh80180824300005.jpeg?Expires=1725318086&Signature=DD55sowgCWwV-F7~E7kRroDG2qUJImyn2~3kyxg7Jbsa60-FDa0d22IxKsSVqDu0HunP4DhHDZhC-SFvcnTdHyv57vbM5lsxm-Qlns32FAi33AQyU0s2ftIqhpGdfLO3CJLhsX--pEumG2Fupw0gcsChoUu6wmJTffF-F0QCRoxXip9lINVpUw~ujx7gRDNbIaCqoHG7JqxbbWMW7JgI7A-9XOBSYo0~ORuzddAFSD0kn3~7uY-DtN2ENouBvgzzVvptLrrcL7xHHCf4TIyueXFCSC8RTlEn0ZK7w28d6qBJ4fivPpS3pG9NL7gE5GsgWeQ~DuTtxcPdXQ3AZORsuQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
ADP dose response for the full platelet model. (A) Relative fluorescence of Fluo-4–loaded human platelets was measured after treatment with 100 nM, 1 μM, or 10 μM ADP. (B-F) After 1 minute of simulated rest, the platelet model was activated by setting the extracellular [ADP] to 100 nM, 1 μM, or 10 μM. Deterministic computation of (B) [Ca2+]i, (C) [IP3], (D) [PLC-β*], (E) deactivated [PLC-β], and (F) activated [Gq·GTP]. (G,H) Gel-filtered human platelets were activated with ADP and maximal changes in (G) [Ca2+]i and (H) [IP3] from basal levels were measured.36 For panels G and H, simulated responses are plotted in red.
