Reduced alkalinization of the phagosomal pH in the absence of NOX2 activity. (A) DC (left) and MØ (right) phagosomal pH kinetics in the presence or absence of 10 μM DPI. The average and standard deviation (SD) of 3 independent experiments (3 different donors) are shown. (B) Control DC (left) and CGD-DC (right) phagosomal pH kinetics after a 15-minute phagocytosis pulse and the indicated time points of chase. Dots represent different blood donors. Slashes show average pH. Statistics were performed using the nonparametric Wilcoxon test for paired data. *P < .05; **P < .01