Th17 are more potent than Th1 cells to induce MMP production by monocytes/macrophages. (A) IL-17, IFN-γ, IL-4; (B) MMP-1, MMP-3, MMP-9; (C) IL-1β, CCL20/MIP-3α, CXCL10/IP-10. T-cell lines were generated as described in the legend of Figure 6. A total of 50 000 T cells were cocultured with equal numbers of allogeneic monocytes for 48 hours. IL-1β, IL-4, IL-17, IFN-γ, MMP-1, MMP-3, and MMP-9 were assessed by multiplex immunoassay, whereas CCL20/MIP-3α and CXCL10 were assessed by ELISA. Bars represent the mean of duplicate cultures; the SD was within 15% of the mean for all conditions. The results are representative of similar data obtained with T cells generated from 2 healthy donors.