Tracking of Foxp3- alloreactive T-cell expansion and differentiation after transplantation and E18 application. (A) CFSE-dye dilution among and IFN-γ production by donor CD4⁺ T cells recovered from spleen (left) or MLN (right) 3 and 6 days after transplantation into lethally irradiated BALB/c mice and 5 days after control mAb (Ctrl, gray shadow) or E18 (black line) application. The figures indicate mean fluorescence intensities (MFI) plus or minus SD of cells selected by the marker. (B) Absolute numbers of donor CD4⁺Foxp3⁻ T cells in spleen (left), MLN (middle), and liver (right) 6 days after transplantation were calculated by multiplying absolute cell number per organ with the percentage of cells as determined by fluorescence-activated cell sorter analysis. Circles represent individual animals. Horizontal bars represent median values with data from 4 experiments being pooled.