Time-dependent induction of E- and P-selectin transcription through VAP-1/SSAO signaling. (A) HUVECs were infected with wild-type (VAP-1) or an enzymatically inactive mutant of VAP-1 (VAP-1 Y471F) and stained with anti–VAP-1 and negative control mAbs for fluorescence-activated cell sorter (FACS) analyses. (B) The transfected cells were analyzed for E-selectin and P-selectin mRNA expression after methylamine administration using real-time PCR. Relative expression levels (means ± SEM) at the indicated time points are shown. Three samples of HUVECs from different individuals were used for each time point. *P < .05