Delivery of Ad5-mOVA in collagen enhances recognition and lysis of OVA-expressing targets in a dose-dependent manner. (A) Mice were immunized subcutaneously with the indicated doses of Ad5-mOVA in PBS or 30 mg/mL collagen in the flank. After 14 days, spleens were harvested for lytic assays as described in “Methods.” Data are representative of 2 experiments with 3 mice per group. (B) Mice were immunized as in panel A, and in vivo CTLs were initiated 13 days later as described in “Methods.” SIINFEKL-loaded splenocyte targets were CFSE-high, and control targets were CFSE-low. Data are representative of 2 experiments of 3 to 5 mice per group. Percentage lysis is the average of both experiments plus or minus SEM. (C) Mice were immunized as in panels A and B, and spleens were harvested after 13 days for 5-hour stimulation with SIINFEKL peptide to determine levels of IFNγ-expressing OVA-specific CD8 T cells as assessed by flow cytometry. Each panel is representative of 3 mice. Lysis is the average plus or minus standard deviation.