Neutrophil depletion during acute inflammation leads to a reduction of sIL6R. (A) Scheme of the air pouch model of local inflammation in C57/BL6 mice as outlined in “Materials and methods.” IL6R neutralization was induced with the anti-mIL6R D7715A7 mAB (100 μg/mouse), which was administered (intraperitoneally) 6 hours before carrageenan injection. Seventy-two hours after carrageenan injection, total cell numbers (B) and cell numbers of neutrophils (C) and mononuclear phagocytes (D) were determined. Values represent the mean (± SD) from 5 different mice (*P ≤ .05). Neutrophil depletion was induced with the Ly6G mAB, which was administered (intraperitoneally) 18 hours before carrageenan injection. Twenty-four hours and 72 hours after carrageenan injection, total cell numbers (E) and cell numbers of neutrophils (F) and mononuclear phagocytes (G) were determined. sIL6R (H) was quantified using ELISA. Values represent the mean (± SD) from 4 different mice (*P ≤ .05).