Coimmunoprecipitation of p53 and Bcl-2. Cells from patient 19 were incubated for 18 hours with no addition, 50 μM chlorambucil, 10 μM nutlin 3a or 10 μM nutlin 3b. CHAPS lysates were prepared and analyzed directly (A), or following immunoprecipitation with anti-Bcl-2 antibodies (B). Immunoprecipitation supernatants were analyzed for Hsp60 to document equal inputs (C).