Effect of EAPB0203 treatment on antiapoptotic and cell-cycle regulatory proteins. (A) Effect of EAPB0203 treatment on the protein levels of the apoptosis regulatory proteins bcl-2, bcl-xl, or the caspase inhibitors c-IAP-1 and c-IAP-2. Cells were treated with EAPB0203 (5 μM) for 24 hours; total SDS protein lysates (50-100 μg/lane) were prepared and immunoblotted as described with specific antibodies. (B) Effect of EAPB0203 on the protein level of p53. Cells were treated with EAPB0203 at the indicated concentration for 24 hours; total SDS protein lysates (50-100 μg/lane) were prepared and immunoblotted with anti-p53 or GAPDH specific antibodies. (C) Effect of EAPB0203 on the protein level of p21. Cells were treated with EAPB0203 at the indicated concentration for 24 or 48 hours; total SDS protein lysates (50-100 μg/lane) were prepared and immunoblotted with anti-p21 or GAPDH specific antibodies.