American Society of Hematology

Figure 6

$Figure 6. Effect of EAPB0203 treatment on mitochondrial membrane potential and cytochrome c release. (A) CEM and HuT-102 cells were treated with EAPB0203 (5 μM) for 24 hours and stained with Rhodamine-123. Fluorescence emission at 525 nm after excitation at 488 nm was quantified by flow cytometer using CellQuest software. Histograms represent dot-plot analysis of Rhodamine-123 fluorescence (x-axis) over FSC (x-axis). Statistics representing the percentage of apoptotic cells with decreased mitochondrial membrane potential are displayed. (B) The HTLV-I–negative CEM and Jurkat, and the HTLV-I–positive HuT-102 and MT-2 malignant T cell lines were treated with EAPB0203 (5 μM) for 24 hours. Apoptotic cytochrome C was measured at the indicated time point in the cytosolic fractions as described in “Measurement of cytochrome c release.” Apoptotic cytochrome c levels were calculated from duplicate measurements and are expressed as percentage increase over control set as 100. Each bar represents the mean plus or minus range (n = 1). Results are representative of 2 independent experiments.$

Effect of EAPB0203 treatment on mitochondrial membrane potential and cytochrome c release. (A) CEM and HuT-102 cells were treated with EAPB0203 (5 μM) for 24 hours and stained with Rhodamine-123. Fluorescence emission at 525 nm after excitation at 488 nm was quantified by flow cytometer using CellQuest software. Histograms represent dot-plot analysis of Rhodamine-123 fluorescence (x-axis) over FSC (x-axis). Statistics representing the percentage of apoptotic cells with decreased mitochondrial membrane potential are displayed. (B) The HTLV-I–negative CEM and Jurkat, and the HTLV-I–positive HuT-102 and MT-2 malignant T cell lines were treated with EAPB0203 (5 μM) for 24 hours. Apoptotic cytochrome C was measured at the indicated time point in the cytosolic fractions as described in “Measurement of cytochrome c release.” Apoptotic cytochrome c levels were calculated from duplicate measurements and are expressed as percentage increase over control set as 100. Each bar represents the mean plus or minus range (n = 1). Results are representative of 2 independent experiments.

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